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If producing v2 sample sheets for devices other than the ones stated, verify the final sample sheet has the i5 index in the proper orientation as described over.
Gary Schroth, PhD provides an introduction to index hopping, explains how to attenuate index hopping concentrations by pursuing library prep ideal methods, and discusses how to remove index-hopped reads applying exceptional dual indexing combos.
Check out how AI is reworking genomic exploration, driving scientific insights, and enabling multiomic discovery
Established A Index Adapters Consult with Index two (i5) Orientation To find out more regarding how to enter i5 bases about the sample sheet in forward or reverse enhance orientation.
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Streamlined RNA-Seq Alternative for very clear and in depth Evaluation through the coding and non-coding transcriptome with Outstanding review flexibility.
If jogging in manual mode and employing bcl2fastq or BCL Transform, or uploading the sample sheet to BaseSpace as being a guide mode operate and attaching a sample sheet, enter the i5 sequence inside the forward path.
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When utilizing BaseSpace Sequence Hub PrepTab, i5 sequences should be entered into these software during the ahead orientation in all conditions and for all instrument kinds.
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